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Wolfenstein: Enemy Territory is a downloadable, free-to-play multiplayer game in which players wage war as Axis or Allies in team-based combat. Its a team game; you will win or fall along with your comrades. The only way to complete the objectives that lead to victory is by cooperation, with each player covering their teammates and using their class special abilities in concert with the others.
We investigated the relationship between the metabolic potential of MAGs and the in situ microbial activity and examined the microbial distribution according to the levels of energy metabolism through analyses of membrane lipids, the biomass-associated enzymes, and the substrate utilization patterns. The highly diverse character of L- and M- vent chimneys was demonstrated by the high abundance of DAGs (56%) and TAGs (12%) among the differentiating metabolites in these two types of chimney (Fig. 3). The microbial community of L- and M- vent chimneys was dominated by Flavobacteriales, Deltaproteobacteriales, Euryarchaeota, and Actinomycetales. The ratios of different categories of carboxylic acids and sugars were all higher in the M- vent community than in the L-vent community, indicating a higher anaerobic respiration capacity in the M- vent community than in the L-vent community. Moreover, the functional gene abundance and the ratios of total substrate utilization were higher in the M- vent community than in the L-vent community, indicating a higher anaerobic respiration capacity in the M- vent community than in the L-vent community. Coenzyme Q10, an ATP synthase component, is a valuable biomarker of anaerobic respiration, and no CoQ10 was detected in the L- vent community, but 5.8 μg/g of CoQ10 was present in M- vent microbial cells. This indicates anaerobic respiration in the M- vent community (Table 1). Fungi were detected in L- but not in M- vent chimneys, which showed a significant correlation with relative abundance of different metabolic groups in the chimney microbiota. These findings together indicate that the M- vent chimney microbial community was more active than the L- vent chimney microbial community. The higher in situ microbial activity in M- vent chimneys is consistent with our previous findings that the M- vent chimney had a higher anaerobic respiration capacity than the L- vent chimney. The L- vent community might be weak in the relationship between the metabolic potential and the diversity of the community in the chimney. Stable isotope probing was used to show that specific sequences of 16S rRNA from the M- vent chimney community shifted toward those from the M- vent community during incubation under different temperatures (Fig. 1). According to this study, the temperature fluctuations in the M- vent chimney clearly affects the microbial community structure by changing the diversity of the community.